A review of the South American metalmark genus Alesa Doubleday, 1847 (Lepidoptera: Riodinidae: Eurybiini) with the description of two new species.

The taxonomy of the South American metalmark butterflies of the genus Alesa Doubleday, 1847 is reviewed. A new taxonomic arrangement for Alesa is supported after the analysis of about 600 specimens, including the recognition of two new species, described herein, A. juliae sp. nov. and A. mariae sp. nov.. Alesa negra Röber, 1931, so far recognized as a junior subjective synonym of A. telephae (Boisduval, 1836), is here recognized as a valid species (stat. rev.) and A. amethystina Gallard & Fernandez, 2015 as a junior subjective synonym (syn. nov.) of A. negra, and A. fournierae Lathy, 1958 is recognized as a junior subjective synonym (syn. nov.) of A. amesis (Cramer, 1777). Neotypes are designated for Papilio amesis Cramer, 1777, Erycina prema Godart, [1824] and Mimocastinia egeria Biedermann, 1936. Lectotypes are designated for Erycina priolas Godart, [1824], Eurybia telephae Boisduval, 1836, A. smaragdifera Westwood, 1851, A. lipara Bates, 1868, A. hemiurga Bates, 1868, A. thelydrias Bates, 1868, and Mimocastnia rothschildi Seitz, 1913. Three previously established species groups are here supported by morphological characters including the following species, "prema group": A. prema, A. esmeralda Salazar & Constantino, 2007, A. beneluzi Gallard & Fernandez, 2015, A. rothschildi and A. juliae sp. nov.; "telephae group": A. telephae, A. humilis Brévignon, 2015, A. negra stat. rev. and A. suzana Ahrenholz & Hall, 2010; and "amesis group": A. amesis, A. mariae sp. nov., A. lipara Bates, 1867, A. hemiurga Bates, 1867 and A. thelydrias Bates, 1867. Adults and their genitalia are illustrated for all species available to examination. The main diagnostic characters used to distinguish the species belonging to the "amesis group" are illustrated, and pictures of adults images in situ and distribution maps are provided. Additionally, all species are diagnosed and discussed based on their morphology.

A new species of Vehilius Godman, 1900 (Lepidoptera, Hesperiidae, Hesperiinae) endemic to the Caatinga, northeastern Brazil: taxonomy, biology and behavior.

A new species belonging to the genus Vehilius Godman, 1900, Vehilius jabre Medeiros, Souza & Kerpel sp. nov., endemic to the Caatinga biome, northeastern Brazil, is here described based on data from adult and immature stages. Information about the biology, hostplant, distribution, and adult and larval behavior of the new species is provided. The new species is morphologically similar to Vehilius warreni (Weeks, 1901), but can be distinguished by a set of characters on wings and male and female genitalia. Adults and genitalia for both sexes of V. jabre sp. nov. and V. warreni are illustrated and compared.

Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America

ABSTRACT Accurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA 'barcode' region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA 'barcodes' to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA 'barcodes', either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA 'barcodes' region alone can be useful for lower level phylogeny in this group, recovering as monophyletic groups such as Copitarsia, Agrotis and Austrandesiita. Conversely, groups such as Mythimna (Mythimna) and the subgenera of Feltia were not recovered as monophyletic, suggesting the need for further taxonomic studies in these groups.

A review of the Neotropical skipper genus Sodalia Evans, 1955 (Lepidoptera: Hesperiidae: Hesperiinae) with the description of a new species.

The Neotropical skipper genus Sodalia Evans, 1955 and its respective species are revised. Sodalia includes five species: S. sodalis (Butler, 1877) (type species), S. argyrospila (Mabille, 1876), S. coler (Schaus, 1902), S. petiti Gaviria-Ortiz, Dolibaina A. Warren sp. nov. (described from low to mid elevations of the western Andes of Ecuador and from the Cordillera de la Costa in Venezuela), and Sodalia spangla (Evans, 1955) comb. nov. hitherto combined with Mnasitheus Godman, 1900. Lectotypes for Pamphila sodalis Butler, 1877 and Achlyodes argyrospila Mabille, 1876 are designated. The genus and species are redescribed and illustrations of relevant morphological characters necessary for identification are provided, as well as updated distributional maps.

Taxonomic revision of the genus Panca Evans, 1955 (Hesperiidae: Hesperiinae: Hesperiini: Moncina) with the description of two new species.

The South American genus Panca Evans, 1955 and its respective species are revised. Previous to this study, the genus included solely its type species, Lerodea subpunctuli Hayward, 1934, until Panca moseri Dolibaina, Carneiro O. Mielke, 2017 was described recently. However, as a result of a broader morphological study including closely related genera, we here propose that Panca assembles 12 species, most of which inhabit open environments such as the Cerrado biome and the natural grasslands of the Atlantic Forest biome. Eight species formerly included among other genera of Moncina are here combined with Panca: Panca satyr (Evans, 1955) comb. nov., Panca tobiasi (Mielke, 1992) comb. nov., Panca trogon (Evans, 1955) comb. nov., Panca steinhauseri (Dolibaina A. Warren, 2015) comb. nov., Panca acroleuca (Plötz, 1884) comb. nov., and Panca mirnae (O. Mielke, Dolibaina, Carneiro A. Warren, 2015) comb. nov. (all formerly in Artines Godman, 1901), Panca paulo (Bell, 1932) comb. nov. (formerly in Eutocus Godman, 1901), and Panca mictra (Evans, 1955) comb. nov. (formerly in Vidius Evans, 1955). Additionally, two new species are described from Brazil: Panca puri Medeiros, O. Mielke Casagrande sp. nov. (from Pará, Pernambuco, Minas Gerais, Rio de Janeiro and Paraná) and Panca xavante Medeiros, O. Mielke Dolibaina sp. nov. (from Mato Grosso, Goiás, Distrito Federal and Minas Gerais). A neotype for Apaustus acroleuca Plötz, 1884 is designated. Illustrations of the male and female genitalia and distribution maps are presented for all the species of Panca and an identification key is provided for both sexes.

Taxonomy of five neglected South American species of Dargida Walker (Lepidoptera: Noctuidae).

The taxonomy of five South American species of Dargida Walker currently allocated in Leucania Ochsenheimer (four species) and in Lasionycta Aurivillius (one species) is revised. Leucania roseilinea (Köhler, 1947), Leucania phaeoneura Hampson, 1913, Leucania mocoides Dognin, 1897, Leucania alboradiata (Hampson, 1905) and Lasionycta radiata (Köhler, 1966), are here combined with the genus Dargida Walker for the first time (comb. nov.). Two new synonyms are proposed, Leucania roseilineoides Poole, 1989 is an objective junior synonym of Borolia roseilinea Köhler, 1947 (syn. nov.) (ICZN 51.4), and B. lilloana Köhler, 1947 is a subjective junior synonym of Leucania phaeoneura Hampson, 1913 (syn. nov.). Lectotype for Borolia lilloana is designated to ensure nomenclatural stability and recognizability of the taxon. All species are redescribed and discussed, and the habitus and male and female genitalia (when available) are given, as well as an updated geographical map.

Taxonomic review of the "Probetoriformes" species group of Symmachia Hübner, [1819] (Lepidoptera: Riodinidae), with the description of two new species from the Brazilian Atlantic Forest.

The "Probetoriformes" species group of Symmachia Hübner, [1819] proposed by Stichel (1910; 1930) is redefined based on morphological evidence, and the species herein included in the group are revised. Two new species from the Brazilian Atlantic Forest are described: S. atlantica Dias, Dolibaina Mielke sp. nov. and S. uirassu Dolibaina, Dias Casagrande sp. nov. Additionally, one restituted status, S. championi Godman Salvin, 1886 stat. rest., one revalidated status, S. belti Godman Salvin, 1886 stat. rev., and one new status, S. meyi Brévignon, 1998 stat. nov., are proposed; therefore, the "Probetoriformes" species group, as herein conceived, includes 10 of the about 60 species of the "catch-all" genus Symmachia. A neotype for Papilio probetor Stoll, 1782 and lectotypes for S. probetor form astiama Seitz, 1913, S. belti and S. championi are designated. The female of the recently described species S. divisora Dolibaina Leite, 2012 is recognized and described for the first time. Additionally, morphologic illustrations and a dichotomous key for species of the "Probetoriformes" species group are provided.

Two new Andean species of Alychna Grishin, 2019 (Hesperiidae, Hesperiinae, Hesperiini, Moncina) from Bolivia and Peru.

Two new species of Alychna Grishin, 2019, Alychna chulumani Medeiros, Dolibaina O. Mielke sp. nov. from Bolivia (La Paz, Chulumani) and Alychna argenteus Medeiros, Dolibaina Casagrande sp. nov. from Peru (Huánuco, Carpish) are described. Illustrations of the wing pattern, forewing stigma and male genitalia of the two species are provided.

Taxonomic revision of the genus Tarmia Lindsey, 1925 stat. rev. (Hesperiidae: Hesperiinae: Hesperiini: Moncina) with the description of a new species from the Andes.

The genus Tarmia Lindsey, 1925, long considered a junior synonym of Phanes Godman, 1901, is here revised and its status is revalidated based on morphological evidence. Two Andean species are included in Tarmia: the type species, T. monastica Lindsey, 1925 comb. rev., and a new species herein described, Tarmia greeneyi A. Warren, Medeiros, Dolibaina O. Mielke sp. nov., from Ecuador, Peru and Bolivia. The male's symmetrical valvae and aedeagus with a twisted median portion, and the very wide ductus bursae of the female genitalia support Tarmia as valid genus apart of Phanes. Illustrations of the main diagnostic characters of both sexes and a distribution map are provided for the species of Tarmia.

Taxonomic revision of Artines Godman, 1901 (Hesperiidae: Hesperiinae: Moncini) with the description of nine new species.

The genus Artines Godman, 1901 and its respective species are revised. The results shows that Artines consists of 15 species distributed from Mexico to Bolivia, Paraguay, southern Brazil and northern Argentina. Nine new species are described: Artines panama Medeiros Carneiro sp. nov. from Panama, Artines solange Medeiros O. Mielke sp. nov. from Brazil (Maranhão), Artines delfos Medeiros Casagrande sp. nov. from Brazil (Amazonas), Artines litoralis Medeiros Dolibaina sp. nov. from Brazil (from Paraíba to Espírito Santo), Artines liege Medeiros, O. Mielke Casagrande sp. nov. from Brazil (central region of Brazil, from Pará to Paraná), Argentina (Misiones) and Paraguay (Alto Paraná), Artines bamba Medeiros O. Mielke sp. nov. from Brazil (Acre and Amazonas) and Peru (San Martin and Madre de Díos), Artines angelica Medeiros sp. nov. from Brazil (Acre, Pará, Maranhão and Mato Grosso), Peru (Madre de Díos) and Bolivia (La Paz), Artines cofus Medeiros Dolibaina sp. nov. from Brazil (Acre and Rondônia) and Peru (Madre de Díos and San Martin) and Artines donia Medeiros Carneiro sp. nov. from Brazil (Rondônia and Goiás). A neotype for Thracides aepitus Geyer, 1832 and a lectotype for Artines atizies Godman, 1901 are designated. Genitalia of both sexes are illustrated and distribution maps for all species are provided. The females of Artines aepitus (Geyer, 1832), Artines focus Evans, 1955 and Artines aquilina (Plötz, 1882) are illustrated for the first time. An identification key is provided for males.

First records of Leucania rawlinsi Adams and L. senescens Möschler (Lepidoptera: Noctuidae) in Brazil: redescription, potential association with Bt maize, larval parasitoids, and spatial and temporal distribution.

Every year the area of transgenic maize planting in Brazil expands, however, our knowledge of the fauna of herbivorous insects associated with this genetically modified crop is restricted. In this work we report for the first time the occurrence of Leucania rawlinsi Adams, 2001 and L. senescens (Möschler, 1890) (Lepidoptera: Noctuidae) in Brazil with larvae feeding on Bt and non-Bt maize silk, as well as their braconid and tachinid larval parasitoids. In order to facilitate the specific identification of these species in future studies, redescriptions of adults including high resolution images are provided. In addition, spatiotemporal distribution data of both species are presented based on systematic surveys at 13 localities in Brazil and the examination of material deposited in several scientific collections. The results are presented and discussed to contribute to the evaluation of the complex of species associated with agricultural systems that include grass crops, especially maize, including Bt varieties.

Taxonomy of the rivorum species-group of Leucania Ochsenheimer, 1816 (Lepidoptera: Noctuidae).

The rivorum species-group of the genus Leucania (Noctuidae: Noctuinae: Leucaniini) is circumscribed and taxonomically revised, with the inclusion of three species. New taxonomic hypotheses were achieved through the morphological analysis of about 250 specimens allied to the type material of L. rivorum Guenée, 1852; L. pampa Schaus, 1894, stat. rev.; and Neleucania multistria Köhler, 1947, stat. rev., the last two resurrected from synonymy with L. rivorum and Dargida lithophilus (Butler, 1882), respectively. Adult habitus and morphology of the genitalia support the transfer of Neleucania multistria to Leucania, comb. nov. Lectotypes for L. rivorum and L. pampa are designated to ensure nomenclatural stability and recognizability of these taxa. Additionally, the similar, rust-colored species Leucania chejela (Schaus, 1921) is compared with species of the rivorum species-group, and is recorded from South America for the first time. All species of the rivorum group are re-described, with high-resolution images of taxonomic characters of the adults (head, patagium, and male and female genitalia), and updated distributional maps presented.

Hidden in plain sight: a morphological study revealing three new species of the skipper genus Drephalys Watson, 1893 (Lepidoptera: Hesperiidae) from Brazil.

Three new species of Drephalys Watson, 1893 from Brazil are described as follows: D. dracarys Madruga, Siewert, Mielke Dolibaina, sp. n. from Acre, Mato Grosso and Rondônia states, D. electrinus Siewert, Madruga, Mielke Dolibaina, sp. n. from Acre and Amazonas states, and D. citrinus Madruga, Siewert, Mielke Casagrande, sp. n. from Amazonas state. Drephalys heraclides Bell, 1942 was reported for the first time from Brazil. To characterize the new species, illustrations of the male genitalia of D. heraclides, D. phoenice (Hewitson, 1867) and D. phoenicoides (Mabille Boullet, 1919) are provided. Diagnosis, adult photos, illustration of the male genitalia and a geographical distribution map are provided for the new species.

Monotypic no longer: a new species of Panca Evans (Lepidoptera: Hesperiidae: Hesperiinae: Moncini) from South America.

A new species belonging to the previously monotypic genus Panca Evans, 1955, P. moseri Dolibaina, Carneiro & O. Mielke sp. nov., from grasslands areas of Peru, Bolivia and Brazil is described. Adults and male and female genitalia of the new species are illustrated, described in detail, and compared with the type species of the genus, Lerodea subpunctuli Hayward, 1934, and with its externally most similar and sympatric species, Vidius mictra Evans, 1955. The systematic position of the genus Panca and its association with lowland open natural habitats of South America are discussed.

Taxonomy of Rufocumbre gen. nov., a new Moncini skipper genus (Lepidoptera: Hesperiidae: Hesperiinae).

Rufocumbre gen. nov. (type species: Rufocumbre celioi sp. nov.), a new genus of Neotropical skippers belonging to the tribe Moncini is described to include five species known only from Brazil. Four new species are described: Rufocumbre celioi sp. nov. from the states of Minas Gerais, Rio de Janeiro, São Paulo, Paraná, Santa Catarina and Rio Grande do Sul; Rufocumbre emeryi sp. nov. from the states of Mato Grosso, Distrito Federal, Goiás and Minas Gerais; Rufocumbre schneideri sp. nov. from the states of Tocantins, Distrito Federal, Minas Gerais and Paraná; and Rufocumbre lucasi sp. nov. from the states of Bahia and Minas Gerais. The taxon Cumbre belli eberti Evans, 1955 is transferred to the new genus and elevated to the species level, Rufocumbre eberti (comb. nov., stat. nov.). Adults, male androconia and genitalia of both sexes are illustrated for all species.

Mesene Doubleday (Lepidoptera: Riodinidae) from Parque Nacional da Serra do Divisor, Acre, Brazil, with taxonomic notes and the description of a new species.

The species of Mesene Doubleday, 1847 collected at the Parque Nacional da Serra do Divisor, Acre, Brazil (PNSD) are listed and supplemented by taxonomic and behavioral notes. In total, 30 specimens were collected, representing seven species including one new: M. monostigma monostigma (Erichson, [1849]), M. nepticula stigmosa Stichel, 1910, M. eupteryx Bates, 1868 stat. rest., M. paraena Bates, 1868, M. epaphus pyrrha Bates, 1868, M. leucophrys Bates, 1868, and M. gracielae sp. nov. The following notes on the taxonomy of Mesene are proposed: M. eupteryx Bates, 1868 stat. rest. is recognized as a valid species, M. epaphus sertata Stichel, 1910 syn. nov. is a new synonym of M. epaphus pyrrha Bates, 1868, and M. lecointrei P. Jauffret & J. Jauffret, 2008 syn. nov. is a new synonym of M. philonis Hewitson, 1874. Mesene margaretta (White, 1843), M. philonis and M. nola are recognized as closely related. Species encountered at the PNSD are illustrated, including the genitalia of males and females, when available.

Argyrogrammana Strand (Lepidoptera: Riodinidae) from Parque Nacional da Serra do Divisor, Acre, Brazil, with the description of four new species.

The species of Argyrogrammana Strand, 1932 collected at the Parque Nacional da Serra do Divisor, Acre, Brazil (PNSD) are listed and detailed with behavioral notes. In total, 17 species were recorded, including 13 new records for Brazil (marked with an asterisk): A. alstonii (Smart, 1979)*, A. nurtia ludibunda Brévignon & Gallard, 1995*, A. pulchra (Talbot, 1929)*, A. bonita Hall & Willmott, 1995*, A. amalfreda (Staudinger, [1877])*, A. physis phyton (Stichel, 1911), A. halli Dolibaina & Dias, sp. nov.*, A. celata Hall & Willmott, 1995*, A. gallardi Dolibaina & Dias, sp. nov.*, A. aparamilla Hall & Willmott, 1995*, A. praestigiosa (Stichel, 1929), A. johannismarci Brévignon, 1995*, A. brevignoni Dolibaina & Dias, sp. nov.*, A. rameli (Stichel, 1930), A. willmotti Dolibaina & Dias, sp. nov.*, A. iracyi P. Jauffret & J. Jauffret, 2007 and A. saulensis tunari Gallard, 2008, comb. nov.*. Four new species are described, two from "amalfreda complex": A. halli Dolibaina & Dias, sp. nov. and A. gallardi Dolibaina & Dias, sp. nov.; one from "trochilia complex": A. brevignoni Dolibaina & Dias, sp. nov.; and one from "stilbe complex": A. willmotti Dolibaina & Dias, sp. nov. Argyrogrammana iracyi saulensis Gallard, 2008 is recognized as a valid species, A. saulensis saulensis, stat. nov., and a new combination is proposed to A. saulensis tunari Gallard, 2008, comb. nov. The females of A. iracyi P. Jauffret & J. Jauffret, 2007 and A. saulensis tunari are recognized and figured for the first time. The taxonomy of the species found at the PNSD is discussed and adults are figured, including illustrations of the genitalia.

Taxonomic review of Gallio Evans, 1955 (Lepidoptera, Hesperiidae, Hesperiinae): one less monotypic genus of Moncini

ABSTRACT Moncini is the tribe of Hesperiidae that comprises the greatest diversity of small, brown, hard to identify skippers. The group is peculiarly classified as having many monotypic genera, thus offering low informative value to its systematics. This study presents a review of the genus Gallio Evans, 1955, a genus formerly recognized as monotypic, and describes three new species, Gallio imperatriz sp. nov. from Maranhão, Brazil, Gallio furtadoi sp. nov. from Mato Grosso, Brazil and Gallio eti sp. nov. from Madre de Díos, Peru and Acre, Brazil (type locality). A lectotype for Vehilius carasta Schaus, 1902 is designated. Gallio is therefore redescribed and illustrations and diagnosis to its species are provided.

Description of two new species of Pheles Herrich-Schäffer, [1853] and notes on the taxonomic position of two species hitherto included in the genus (Riodinidae: Riodininae).

Two new species of Pheles Herrich-Schäffer, [1853], Pheles plaumanni Dolibaina & Dias sp. nov. and Pheles obscura C. Mielke, Dolibaina & Dias sp. nov. are described from southern Brazil. Through the comparative study of the morphology of the head, labial palpus and the genitalia two species are removed from Pheles and the following new combinations are proposed: Pachythone bicolor (Godman & Salvin, 1886) comb. nov. and Melanis caatingensis (Callaghan & Nobre, 2014) comb. nov. The species studied are pictured and details of the head and genitalia are illustrated.

New species and taxonomic notes on Anteros Hübner, [1819] and Ourocnemis Bethune-Baker, 1887 (Lepidoptera: Riodinidae).

Three new species of Anteros Hübner, [1819] from south and southeastern Brazil are described: A. aliceae Dias & Siewert, sp. nov., A. zikani Siewert, Dias & Dolibaina, sp. nov. and A. ethani Dias, Dolibaina & Mielke, sp. nov. Habitus and genitalia of male and female specimens of the new species are illustrated, along with illustrations of the habitus and male genitalia of the most similar species for proper distinction. The following taxonomic changes are proposed: A. micon Druce, 1875, stat. rest., A. roratus Godman & Salvin, 1886, stat. rest., and A. theleia Stichel, 1910, stat. nov. Anteros formosus stramentarius Stichel, 1909, syn. nov. is sunk as a junior subjective synonym of A. formosus. Additionally, Ourocnemis axiochus (Hewitson, 1867), stat. rest., is recognized as a species distinct from O. archytas (Stoll, 1787); illustrations of the male genitalia and of the differential characters of the wing pattern of both species are provided. A male of the rare Ourocnemis boulleti Le Cerf, 1911, is reported from southeastern Peru. Distributional data and a map are provided for all species studied.